What Does CIEF Stand For?

CIEF stands for Capillary Isoelectric Focusing

CIEF, or Capillary Isoelectric Focusing, is an advanced analytical technique used for the separation and characterization of biomolecules, particularly proteins and peptides, based on their isoelectric points. This method involves the application of a pH gradient within a narrow capillary, allowing charged molecules to migrate until they reach a pH that corresponds to their isoelectric point, at which they become neutral and stop moving. CIEF is renowned for its high resolution, efficiency, and ability to analyze complex mixtures, making it a valuable tool in proteomics, biopharmaceutical development, and clinical diagnostics.