What Does ELISA Stand For?

ELISA stands for Enzyme Linked Immunosorbent Assay

ELISA, or Enzyme Linked Immunosorbent Assay, is a widely used biochemical technique designed to detect and quantify specific proteins, antibodies, or hormones in a sample. This assay utilizes antigen-coated wells, where a sample is applied, allowing the target molecules to bind to the antigens. Subsequently, an enzyme-linked secondary antibody specific to the target is introduced, followed by a substrate that reacts with the enzyme to produce a measurable signal, typically a color change. ELISA is essential in various fields, including medical diagnostics, research, and quality control, due to its sensitivity, specificity, and ease of use.