What Does HEPES Stand For?

HEPES stands for N 2 Hydroxyethylpiperazine N 2 Ethanesulfonic Acid

HEPES, or N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, is a zwitterionic buffering agent widely used in biological and biochemical research. It maintains physiological pH in cell culture and various laboratory experiments, providing stability under a range of temperature and ionic conditions. HEPES is highly soluble in water and is preferred for its minimal interaction with biological systems, making it suitable for applications involving proteins, cells, and enzymes.