What Does HILIC Stand For?

HILIC stands for Hydrophilic Interaction Chromatography

HILIC, or Hydrophilic Interaction Chromatography, is a chromatographic technique used for the separation of polar and hydrophilic compounds. It operates on the principle of interactions between the sample molecules and a hydrophilic stationary phase, facilitating the separation based on differences in polarity and solubility. HILIC is particularly effective for analyzing biomolecules such as amino acids, sugars, and other polar compounds, making it a valuable method in fields such as biochemistry, pharmaceuticals, and environmental analysis. The technique often employs organic solvents as mobile phases, which enhances separation efficiency and sensitivity for hydrophilic analytes.