What Does SILAC Stand For?

SILAC stands for Stable Isotope Labeling By Amino Acids In Cell Culture

SILAC, or Stable Isotope Labeling By Amino Acids In Cell Culture, is a quantitative proteomic technique used to study protein dynamics and interactions within living cells. By incorporating stable isotope-labeled amino acids into cell culture, researchers can differentiate between proteins synthesized in the presence of labeled versus unlabeled amino acids. This method enables precise measurement of protein abundance and turnover, providing insights into biological processes and disease mechanisms while minimizing sample handling and variability. SILAC is widely utilized in various fields, including cancer research, metabolism, and drug development.