What Does TIRFM Stand For?

TIRFM stands for Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy (TIRFM) is a sophisticated imaging technique that utilizes the principle of total internal reflection to excite fluorophores near a sample-substrate interface. This method enhances the detection of fluorescent signals from a limited region close to the surface, improving spatial resolution and allowing for the observation of dynamic processes at the molecular or cellular level. TIRFM is widely used in biological and biomedical research for studying membrane interactions, molecular dynamics, and live cell imaging.